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Genes identified from PB-based in vivo genetic screen of cortical development

Journal: Nature Communications

Article Title: Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice

doi: 10.1038/s41467-018-04880-8

Figure Lengend Snippet: Genes identified from PB-based in vivo genetic screen of cortical development

Article Snippet: The concentration of primary antibodies: NeuN (1:500, Millipore, Cat.# MAB377), anti-Cux1 rabbit polyclonal antibody (1:500; Santa Cruz Biotechnology, sc-13024), anti-5-bromo-2′-deoxyuridine (BrdU) rat monoclonal antibody (1:250; Abcam, Cat.# ab6326), anti-Ki67 rabbit polyclonal antibody (1:500; Millipore, AB9260), anti-Dmd rabbit polyclonal antibody (1:500, Proteintech, Cat. #:12715-1-AP), anti-Rab27b rabbit polyclonal antibody (1:500, Proteintech, Cat. #:13412-1-AP), anti-Dmghd rabbit polyclonal antibody (1:500, Proteintech, Cat. #:24813-1-AP), anti-Hapcal rabbit polyclonal antibody (1:500, Proteintech, Cat. #:10989-1-AP), anti-Rnf166 rabbit polyclonal antibody (1:500, ABclonal, Cat. No.: A8276), anti-Cep152 rabbit polyclonal antibody (1:500, Proteintech, Cat. #: 21815-1-AP), anti-Ick rabbit polyclonal antibody (1:500, Proteintech, Cat. #:13611-1-AP), anti-Stx1b mouse monoclonal antibody (1:500, Proteintech, Cat. #: 66437 − 1-lg), anti-Vps13b rabbit polyclonal antibody (1:500, Proteintech, Cat. #: 24505-1-AP), anti-Med13l rabbit antibody (1:500, AVIVA SYSTEM BIOLOGY, Cat. #: OASG07310).

Techniques: In Vivo, Sequencing, Control, Migration, Residue, Phospho-proteomics, Activation Assay, Activity Assay, Translocation Assay, Modification, Ubiquitin Proteomics